Sysmex公司XN-1000v全自動動物血液分析儀在魚類上的應用
Abstract
Hematological studies provide essential information about the health of animals, which is crucial for veterinary medicine, scientific research, and aquaculture. Automatic hematological analyzers are an alternative to manual methods, offering faster and more reliable results. The objective of this study was to validate the Sysmex XN-1000V automatic hematology analyzer for blood samples from rainbow trout (Oncorhynchus mykiss), examine the effects of two anticoagulants (K2EDTA and lithium heparin), and establish normal blood reference values for this fish species. Additionally, comparative studies were conducted between the Sysmex XN-1000V and manual methods (hemocytometer cell count and blood smear estimation), and reference intervals were established. Ninety-nine heparinized blood samples were analyzed for validation and sample stability tests. The results showed extremely good precision, with a coefficient of variation (CV) below 3% for RBCs, HGB, and HCT and less than 5% for non-RBC cells (leukocytes plus thrombocytes). However, heterophils (%) exhibited higher variability, with a CV of 15.08%. Linearity was excellent, and the carry-over was below 1% for all parameters. The sample stability test indicated that samples could be analyzed for up to 48 h when stored at 4 ?C and up to 24 h at room temperature. Non-RBC cells were the first to degrade over time. The automated and manual methods demonstrated good correlation and agreement, validating the analyzer’s accuracy. The effects of two anticoagulants, K2EDTA and lithium heparin, on the blood samples were also studied. Heparin was the preferred anticoagulant for routine hematological analysis of rainbow trout blood with the Sysmex XN-1000V analyzer. In conclusion, the Sysmex XN-1000V enables complete hemogram analyses to be performed quickly and accurately, standardizing techniques, harmonizing results, and providing reliable reference intervals with O mykiss blood.
摘要：
血液學研究提供了有關動物健康的重要信息，這對獸醫、科學研究和水產養殖至關重要。自動血液分析儀是手動方法的替代品，可提供更快、更可靠的結果。本研究的目的是驗證Sysmex XN-1000V全自動血液分析儀對虹鱒（Oncorhynchus mykiss）血液樣本的檢測效果，檢查兩種抗凝劑（K2EDTA和肝素鋰）的效果，并為該魚類建立正常的血液參考值。此外，對Sysmex XN-1000V和手動方法（血細胞計數器細胞計數和血涂片估計）進行了比較研究，并建立了參考區間。對99份肝素化血液樣本進行了驗證和樣本穩定性測試分析。結果顯示，精確度極高，紅細胞、血紅蛋白和紅細胞壓積的變異系數（CV）低于3%，非紅細胞（白細胞和血小板）的變異系數低于5%。然而，異質性（%）表現出更高的變異性，變異系數為15.08%。線性非常好，所有參數的結轉率均低于1%。樣品穩定性測試表明，在4℃下儲存時，樣品可以分析長達48小時?在室溫下持續24小時。非紅細胞是第一個隨著時間的推移而降解的細胞。自動和手動方法顯示出良好的相關性和一致性，驗證了分析儀的準確性。還研究了兩種抗凝劑K2EDTA和肝素鋰對血液樣本的影響。肝素是Sysmex XN-1000V分析儀對虹鱒血進行常規血液學分析的首選抗凝劑。總之，Sysmex XN-1000V能夠快速準確地進行完整的血象分析，規范技術，協調結果，并為O mykiss血液提供可靠的參考區間。
關鍵詞：XN-1000v全自動動物血液分析儀，魚類健康；血液學；血液學方法驗證；血液自動分析儀；虹鱒魚
